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KMID : 1143420160090501019
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Public Health Weekly Report
2016 Volume.9 No. 50 p.1019 ~ p.1023
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Characteristics of vancomycin-intermediate Staphylococcus aureus (VISA) isolates in Korea, 2015
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Lim Gyo-Jin
Kim Il-Hwan
Park Chan
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Abstract
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BACKGROUND: Vancomycin is being used for treatment of methicillin-resistant Staphylococcus aureus (MRSA), but vancomycin-intermediate S. aureus (VISA) emerged and was reported in Japan in 1996. Since then, VISA and vancomycin-resistant S. aureus (VRSA) have become growing concerns worldwide. In the present study, we investigated the characteristics of VISA isolated in Korea in 2015.
METHODOLOGY: Confirmatory tests for vancomycin resistance were performed on S. aureus isolates procured from tertiary hospitals that participated in the Sentinel Surveillance System for VRSA and VISA in 2015. VISA isolates were confirmed by vancomycin MIC values of agar dilution, broth microdilution and Etest according to the CLSI guideline (M100-S24, 2015). Molecular characterization was performed using MLST, SCCmec, agr, rep-PCR typing, and toxin genes, including, toxic shock syndrome toxin-1 (TSST-1) and enterotoxins.
RESULTS: Thirty-five out of 53 referred isolates were identified as VISA isolates with vancomycin MIC of 3 mg/L or more. The isolates were characterized as follows: ST5-SCCmec II-agr II with enterotoxin genes of sec-seg-sei (31 isolates), sea-sec-seg-sei (3 isolates), and negative (1 isolate), respectively. TSST-1 gene was detected in all VISA isolates. Rep-PCR typing showed that 34 VISA strains belong to the same cluster (>95% homology).
CONCLUSION: In 2015, 35 VISA strains were isolated in Korea, but VRSA was not detected. All VISA isolates showed ST5-SCCmec II-agr II type patterns.
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KEYWORD
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